Kligler iron agar kia combines features of kligler s lead acetate medium and russells double sugar agar. Microbiologie cours anamicrobiologie cours analyse. Microbiologie industrielle introduction suite remarques. Bacteriologie medicale, techniques usuelles request pdf. Publication date 1897 topics roussel, georges, microbiology publisher paris collection. The basal medium of kia is composed of casein and meat peptones with the addition of lactose and dextrose. Salmonella shigella ss agar composition, principle, uses. References normatives microbiologie des aliments nf en iso 10273 decembre 2003. Dictionnaire des techniques microbiologie technique crdp daquitaine voir.
Voir plus didees sur le theme microbiologie, bacterie et genie biologique. The production of acid by lactose andor dextrose fermentation results in color. Kligler iron agar is used for the identification of enterobacteria by the rapid detection of lactose and glucose fermentation with or without gas production, as well as the production of hydrogen sulfide. Ensemencement dune galerie didentification simplifiee en tubes voir fiches techniques.
Ss agar is a modification of the desoxycholate citrate agar. Baixe no formato pdf, txt ou leia online no scribd. Kliglerhajna gelose 3555378 lactoseglucosehs 3564844. It is recommended for testing clinical specimens and food testing for the presence of salmonella spp.
Les bacteries acquierent les nutriments par diffusion passive a travers leur paroi cellulaire. Le milieu incuber 18 24 contient du thiosulfate h 37c pouvant donner du h2s. Appliquer les connaissances acquises grace a ce cours a lenseignement des. Sfa measurement of ph value of blood pdf sciences fondamentales appliquees. History in 1911, russell described a medium containing two sugars for the isolation of typhoid bacilli in urine. Inoculate tsi agar by first stabbing through the center of the medium to the bottom of the tube and then streaking on the surface of the agar slant.
Kligler iron agar kia combines features of kliglers lead acetate medium and russells double sugar agar. Kligler iron agar intended use kligler iron agar is used for the identification of enterobacteria by the rapid detection of lactose and glucose fermentation with or without gas production, as well as the production of hydrogen sulfide. Donnez deux raisons specifiques pour lesquelles cela est vrai. Kligler iron agar kia or the identificaiton of enteric. If lactose or sucrose is fermented, a large amount of. Leave the cap on loosely and incubate the tube at 35c in ambient air for 18 to 24 hours. Le comptage des colonies bacteriennes sest fait 24. Dec 11, 2018 salmonella shigella ss agar is moderately selective and differential medium for the isolation, cultivation and differentiation of salmonella spp. Tsi agar is used for the determination of carbohydrate the butt of the tube because it is under lower oxygen tension. Bacteriologie generale ue optionnelle du groupe b du semestre 5 niveau l3 biologie materiel et hygienesecurite. The best sleeping position for back pain, neck pain, and sciatica tips from a physical therapist duration. Grace a une tryptophanase, les bacteries peuvent degrader le tryptophane en indole, acide pyruvique et nh 3.